We are investigating the ability of NK cells to recognize and kill microbes, mostly fungi, but also bacteria. Currently, we are working on the fungal ligand, NK receptors and their downstream signal transduction, the NK-microbial synapse, granule trafficking and degranulation. Most of the work we do is in human.
We have a mAb library to surface molecules of YT cells. We use inhibitors and knockdown to study signaling and receptors. We use atomic force microscopy to study the synapse and cytoskeleton of NK cells. We use live cell imaging to study conjugate, synapse, granule trafficking. We use intravital imaging of the mouse lung to study NK cell (and other cells).
Would love to have some help developing mass cytometry for our studies of NK cells